Are AMPure beads SPRI beads?
The Agencourt AMPure XP PCR* purification system utilizes Beckman Coulter’s solid-phase reversible immobilization (SPRI) paramagnetic bead technology for high-throughput purification of PCR amplicons. The result is a more purified PCR product.
What is AMPure beads?
Polystyrene – magnetite beads (Ampure) are coated with a layer of negatively charged carboxyl groups. DNA’s highly charged phosphate backbone makes it polar, allowing it to readily dissolve in water (also polar). Changing the amount of PEG and salt concentration can aid in size selecting DNA (2).
How do AMPure beads bind to DNA?
Each bead is made of polystyrene surrounded by a layer of magnetite, which is coated with carboxyl molecules. It is these that reversibly bind DNA in the presence of the “crowding agent” polyethylene glycol (PEG) and salt (20% PEG, 2.5M NaCl is the magic mix).
What does SPRI stand for beads?
Solid Phase Reversible Immobilization (SPRI) Methodology SPRI technology uses paramagnetic beads to selectively bind nucleic acids by type and size, and are used for high-performance isolation, purification, and cleanup protocols.
Can you substitute Ampure XP for SPRI beads?
The bead mixes described in this protocol are drop-in substitutes for AMPure XP and RNAClean XP beads (Beckman Coulter), but at about 1/100 of the cost (~$0.55/mL vs. $15–$70/mL at current Canadian prices). Prepare at least 10 mL 1 N HCl in a glass bottle from available concentrated stock.
What should I compare my SPRI bead mix to?
They can be compared to AMPure XP or RNAClean XP, or to a previous batch of homemade mix.
What’s the difference between Ampure XP and spriselect?
According to their data sheets Ampure XP is used for PCR product purification while SPRIselect is used for size selection. However I know that many people use AMPure XP for double sided size selection. Does somebody knows what is the difference between AMPure XP and SPRIselect?
What are the benefits of Ampure XP primers?
AMPure XP is the Gold Standard for Bead Based, Next-Generation Sequencing (or NGS) Cleanup 1 High recovery of amplicons greater than 100bp 2 Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants 3 Predictable and consistent size selection More