What is SimPlate?
Multiple Enzyme Technology Medium—the SimPlate for HPC medium uses IDEXX Multiple Enzyme Technology (MET) to detect heterotrophic plate count (HPC) bacteria in water. The reagent contains multiple unique enzyme substrates, each targeting a different bacterial enzyme.
How do you calculate heterotrophic count?
To compute the heterotrophic plate count for pour plate, spread plate, and membrane filter methods (CFU/mL), divide either the total or average number of colonies per plate by the sample volume. (Use the average number of colonies if duplicate plates of the same dilution.)
Is heterotrophic plate count same as total plate count?
Colony counts of heterotrophic bacteria, referred to as HPC, provide an indication of the general load of aerobic and facultative anaerobic bacteria of a water sample. This indicator is also known as standard plate count (SPC), aerobic plate count (APC) and total plate count (TPC).
What is the heterotrophic Plate Count ( HPC )?
Applications. The heterotrophic plate count (HPC), formerly known as the standard plate count, is a procedure for estimating the number of live, culturable heterotrophic bacteria in water and for measuring changes in swimming pools or during water treatment and distribution. Colonies may arise from pairs, chains, clusters,
Can a Simplate be used for plate count agar?
Easy-to-count wells on a round plate that correlates to plate count agar. Positives fluoresce under UV light. The SimPlate for HPC test is U.S. EPA approved and included in Standard Methods for Examination of Water and Wastewater. Standard method for SimPlate.
What can Simplate be used for in HPC?
SimPlate® for HPC (IDEXX) The US EPA has approved SimPlate for compliance testing of drinking water. It is a new testing method for heterotrophic plate count that eliminates the need for medium preparation and produces easy to use results. SimPlate has significant benefits compared to traditional pour plates:
How are heterotrophic bacteria detected on the Simplate?
The heterotrophic bacteria are detected as blue-fluorescent wells on the SimPlate. Count the number of positive wells and look up the corresponding MPN value using the table provided. Reanalyze samples containing more than 738 MPN/ml, by diluting in sterile deionized water or phosphate buffer.