What is the molecular weight of GFP?

What is the molecular weight of GFP?

27 kD
The GFP cDNA consists of 730 bp, which encode a 238 amino acid protein with a molecular weight of 27 kD (2). Wild-type GFP emits a vibrant green fluorescence upon exposure to blue light (450-490 nm).

What is the kDa of GFP?

27 kDa
The green fluorescent protein (GFP), originally discovered in the jellyfish, Aequorea victoria, is composed of 238 amino acids and has a molecular weight of 27 kDa.

Is GFP a monomer?

GFP is a 26.9 kDa monomeric 11-strand beta barrel and like many beta barrels it is extremely thermodynamically stable [14–17]. Enhanced GFP (eGFP) has been mutated to further increase its quantum yield and fluorescence extinction co-efficiency (ε) over standard GFP [18,19].

What are the properties of GFP?

Green fluorescent protein (GFP) is a protein in the jellyfish Aequorea Victoria that exhibits green fluorescence when exposed to light. The protein has 238 amino acids, three of them (Numbers 65 to 67) form a structure that emits visible green fluorescent light.

Where does the green fluorescent protein GFP come from?

Green fluorescent protein. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm.

What is the molecular formula for glucose 6 phosphate?

Glucose 6-phosphate PubChem CID 5958 Structure Find Similar Structures Molecular Formula C6H13O9P Synonyms Glucose 6-phosphate Robison ester D-gluc Molecular Weight 260.14

What is the quantum yield of green fluorescent protein?

The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy…

How is GFP used to analyze colocalization of proteins?

GFP can be used to analyse the colocalization of proteins. This is achieved by “splitting” the protein into two fragments which are able to self-assemble, and then fusing each of these to the two proteins of interest. Alone, these incomplete GFP fragments are unable to fluoresce.