What can confocal microscopy be used for?

What can confocal microscopy be used for?

Confocal microscopy provides the capacity for direct, noninvasive, serial optical sectioning of intact, thick, living specimens with a minimum of sample preparation as well as a marginal improvement in lateral resolution compared to wide-field microscopy.

What is a confocal microscope best used for?

Confocal microscopes come in different flavors, but they all have in common the application of non-invasive optical sectioning at low radiation damage, which is ideal for studying structural and functional properties of living cells at full 3D microscopic resolution.

What does confocal microscopy measure?

Confocal microscopy is an optical method for obtaining three-dimensional images of surface textures and objects. With a reflected-light method, the roughness of a surface can be accurately measured without contact. The technically similar method of laser scanning fluorescence microscopy is used mainly in microbiology.

What are laser scanning confocal microscopes used for?

Laser scanning confocal microscopy (usually shortened to just confocal microscopy) uses the principle of fluorescence excitation to investigate the structural properties of cells and the location of particular structures or protein populations within those cells in fixed tissue.

What are the applications of laser scanning confocal microscopy?

The broad range of applications available to laser scanning confocal microscopy includes a wide variety of studies in neuroanatomy and neurophysiology, as well as morphological studies of a wide spectrum of cells and tissues.

How is confocal microscopy used to probe cellular structure?

Today, confocal microscopy, when coupled to the application of new advanced synthetic fluorophores, fluorescent proteins, and immunofluorescence reagents, is one of the most sophisticated methods available to probe sub-cellular structure.

Can a confocal microscope be used for multiphoton imaging?

A confocal laser scanning microscope must be modified and equipped with a specialized near-infrared pulsed laser system in order to perform multiphoton imaging. The following characteristics should be used as a guide when comparing multiphoton excitation with conventional laser scanning confocal microscopy.

What is the ops code for confocal scanning microscopy?

OPS-301 code. 3-301. Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser confocal scanning microscopy (LCSM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out-of-focus light in image formation.