Is polybrene stable?

Polybrene solution is stable for 2 months at +4°C.

What do you dilute polybrene in?

First you need to dissolve it in water or 150mM NaCl to create a stock solution. Make sure you sterile filter this under a hood. In your case, you should make a stock solution at 6mg/mL so that it’s an easy 1:1000 dilution when you are adding to your cells.

Can polybrene be filtered?

(10 mg/ml) Dissolve Polybrene (Aldrich) at a concentration of 10 mg/ml in H2O and sterilize the solution by passing it through a 0.22-μm filter. Store the solution as small aliquots (0.25-ml) at -20°C until needed.

Is polybrene toxic to cells?

Polybrene is considered non-toxic at low concentrations, but has been found to negatively affect cell proliferation in some cell types, such as keratinocytes, at concentrations greater than 10 µg/mL [16].

Can You dilute Polybrene to a stock solution?

In your case, you should make a stock solution at 6mg/mL so that it’s an easy 1:1000 dilution when you are adding to your cells. Polybrene is stable for 2 months at 4 degC but it’s best to aliquot the rest and freeze at -20 degC.

What should the concentration of Polybrene be in a cell?

The optimal concentration of Polybrene® depends on cell type and may need to be empirically determined (usually in the range of 2–10 µg/ml). Excessive exposure to Polybrene® (>12 hr) can be toxic to some cells.

What’s the best way to store Polybrene stock?

Required Solutions Polybrene Stock is 10,000x in medium stored at 4°C (40 mg/ml) and user stock is 100x. Final concentration is 4 µg/ml. Medium for Phoenix cells: DMEM, 10% FBS, 1% Non-essential amino acids, 1% Pen-Strep, 1% Glutamate Store medium at 4°C, warm to 37°C before use. 2.5 M CaCl2 Filter sterilize and store aliquots at -20°C 2x HBS