## How do I make a 1 10000 dilution?

Another way is to dilute the stock 1/10 twice and then perform a further 1/100 dilution: 1/10 x 1/10 x 1/100 = 1/10,000 dilution This would yield 100 ml of a 1/10,000 dilution of stock in water.

## How do you make a 1 in 200 dilution?

1 ml of serum + 199 ml of diluent = 1/200 dilution.

**How do you calculate the dilution of a dilution?**

The dilution factor or the dilution is the initial volume divided by the final volume. For example, if you add a 1 mL sample to 9 mL of diluent to get 10 mL of solution, DF=ViVf = 1mL10mL=110 . This is a 1:10 dilution.

### How to prepare 1.0 ml of 1 : 1000 dilution?

For example, to prepare 1.0 ml of a 1:1000 dilution, first prepare 1:10 dilution in100 μl volume (10 μl + 90 μl), and then prepare a 1:100 dilution in 1.0 ml volume by using 10 μl of an intermediate 1:10 dilution (10 μl + 990 μl). 5. Proper pipette usage

### How to calculate the volume of a dilution solution?

Multiply the final desired volume by the dilution factor to determine the needed volume of the stock solution. In our example, 30 mL x 1 ÷ 20 = 1.5 mL of stock solution.

**How to prepare a volume of highly diluted antibody?**

In order to prepare a small volume of a highly diluted antibody solution, it may be necessary to make it in two steps. For example, to prepare 1.0 ml of a 1:1000 dilution, first prepare 1:10 dilution in100 μl volume (10 μl + 90 μl), and then prepare a 1:100 dilution in 1.0 ml volume by using 10 μl of an intermediate 1:10 dilution (10 μl + 990 μl).

#### Is the dilution-fold the same in each step?

Since the dilution-fold is the same in each step, the dilutions are a geometric series (constant ratio between any adjacent dilutions). For example: 1/3, 1/9, 1/27, 1/81 Notice that each dilution is three-fold relative to the previous one. In four dilutions, we have covered a range of 181/3 = 60-fold.